Ribosome Rescue and Translation Termination at Non-Standard Stop Codons by ICT1 in Mammalian Mitochondria
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چکیده
منابع مشابه
Response to “Ribosome Rescue and Translation Termination at Non-standard Stop Codons by ICT1 in Mammalian Mitochondria”
Release factors (RFs) govern the termination phase of protein synthesis. Human mitochondria harbor four different members of the class 1 RF family: RF1Lmt/mtRF1a, RF1mt, C12orf65 and ICT1. The homolog of the essential ICT1 factor is widely distributed in bacteria and organelles and has the peculiar feature in human mitochondria to be part of the ribosome as a ribosomal protein of the large subu...
متن کاملResponse to the Formal Letter of Z. Chrzanowska-Lightowlers and R. N. Lightowlers Regarding Our Article “Ribosome Rescue and Translation Termination at Non-Standard Stop Codons by ICT1 in Mammalian Mitochondria”
Most deviations from the universal genetic code exist in the mitochondrial translation system. In human mitochondria, two arginine codons, AGA and AGG, have no cognate tRNAs; mtDNA-encoded cytochrome c oxidase subunit I (MTCO1) and NADH dehydrogenase 6 (MTND6) carry AGA and AGG codons at the end of their mRNAs, respectively. We previously demonstrated in vitro the possible engagement of ICT1 in...
متن کاملHuman Cells Require Non-stop Ribosome Rescue Activity in Mitochondria
Bacteria use trans-translation and the alternative rescue factors ArfA (P36675) and ArfB (Q9A8Y3) to hydrolyze peptidyl-tRNA on ribosomes that stall near the 3' end of an mRNA during protein synthesis. The eukaryotic protein ICT1 (Q14197) is homologous to ArfB. In vitro ribosome rescue assays of human ICT1 and Caulobacter crescentus ArfB showed that these proteins have the same activity and sub...
متن کاملICT1 comes to the rescue of mitochondrial ribosomes.
In the current issue, Richter et al (2010) show that mammalian mitochondrial ribosomes contain a ribosomal protein (ICT1) that acts as a ribosome-dependent, codonindependent peptidyl-tRNA hydrolase. This ribosomal protein can rescue ribosomes stalled on mRNAs lacking a termination codon. Every translational system runs into trouble when it encounters mRNAs that have lost the translation termina...
متن کاملDifferences of Translation Termination Sites Among the Three Stop Codons
Most organisms use different mechanisms of translation termination for the three types of stop codons, i.e. UAA, UAG, and UGA [1]. Using the GenBank database, we have conducted comprehensive computer analyses of translation termination sites in order to find the difference of consensus sequence patterns around each type of stop codons. We also systematically analyzed the preference of stop codo...
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ژورنال
عنوان ژورنال: PLoS Genetics
سال: 2014
ISSN: 1553-7404
DOI: 10.1371/journal.pgen.1004616